Ovalbumin is an elastase substrate.

Ovalbumin is an elastase substrate.

Ovalbumin is partially homologous in sequence with the proteinase inhibitors alpha 1-proteinase inhibitor and anti-thrombin III. The region of sequence in ovalbumin which corresponds to the reactive sites of these proteinase inhibitors is susceptible to attack by subtilisin, elastase, thermolysin, bromelain, and Bacillus cereus protease. The esterase activity of elastase is not inhibited by ova...

Elastase substrate specificity tailored through substrate-assisted catalysis and phage display.

The catalytic histidine of human neutrophil elastase was replaced with alanine (H57A) to determine if a substrate histidine could substitute for the missing catalytic group-'substrate-assisted catalysis'. H57A and wild-type elastase were recovered directly from Pichia pastoris following expression from a synthetic gene lacking the elastase pro sequence, thereby obviating the need for zymogen ac...

Soluble Ovalbumin In Vivo Cross-Presented Much More Efficiently than Cell-Associated Ovalbumin Is

Ovalbumin is synthesized in mouse cells transformed with the natural chicken ovalbumin gene.

The entire chicken ovalbumin gene, accompanied by genomic DNA sequences flanking both termini of the gene and three copies of the herpes simplex virus thymidine kinase gene, has been cloned in plasmid pBR322. This recombinant plasmid was linearized and used to transform thymidine kinase-deficient mouse cells. Thymidine kinase-positive transformants were selected by their ability to grow in the ...

Is Porcine Pancreatic Elastase a Suitable Model for Human Leukocyte Elastase?

Human Leukocyte Elastase (HLE) has recently been implicated as a poor prognostic marker in certain breast cancers and as a key component involved in Chronic Obstructive Pulmonary Diseases. Because HLE is so expensive, Porcine Pancreatic Elastase (PPE) has been used to model elastase activity and inhibition. This paper elucidates the differences and similarities between HLE and PPE through enzym...